In a recent article in Nature, published on-line 3rd June, PhD student Marina Vietri and her co-workers in Harald Stenmark’s group at Centre for Cancer Biomedicine and Institute for Cancer Research have uncovered a new cellular mechanism that contributes to keep our genome intact.
The authors of the Nature paper. Front row, from left: Kay O. Schink, Harald Stenmark, Marina Vietri (first author), Coen Campsteijn (co-corresponding author). Middle row, from left: Camilla Raiborg, Liliane Christ, Sebastian W. Schultz. Back row, from left: Andreas Brech, Catherine Sem Wegner, Sigrid B. Thoresen. Photo: Tore Skotland.
This is the second article in Nature the group has published the last two months – you may read the other article here.
NRK Viten has published this article about the research: Norske forskarar har avdekka mysterium bak celledeling
The research by Vietri and her colleagues explains how the cell protects the DNA from damage during cell division: During cell division, the nuclear envelope breaks down so that duplicated chromosomes can be separated by the microtubule-containing spindle apparatus. Upon completion of this process, in anaphase, new nuclear envelopes are formed around the two daughter nuclei, and the mitotic spindle is disassembled by a mechanism that has not been known.
Vietri and her co-workers noticed that certain subunits of a protein complex known as endosomal sorting complex required for transport (ESCRT) accumulate around the reforming daughter nuclei in anaphase. This observation made them uncover a mechanism whereby ESCRT proteins coordinate nuclear envelope sealing and mitotic spindle disassembly. The ESCRT proteins are recruited to points in the reforming nuclear envelope that are intersected by microtubules. Here, they recruit an enzyme, Spastin, that severs microtubules. The remaining holes in the nuclear envelope are then sealed by the membrane-healing activity of the ESCRT proteins.
Vietri and co-workers also addressed what happens if this process goes wrong. By interfering with normal ESCRT functions during anaphase, the researchers observed that DNA becomes damaged, so evidently the novel mechanism of spindle disassembly and nuclear envelope sealing is important for keeping our genome safe.
Because genome instability is strongly connected to cancer development, it will now be interesting to examine which roles the ESCRT machinery play in preventing cancer.
Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing
Marina Vietri, Kay O. Schink, Coen Campsteijn, Catherine Sem Wegner, Sebastian W. Schultz, Liliane Christ, Sigrid B. Thoresen, Andreas Brech, Camilla Raiborg & Harald Stenmark
Nature (2015) doi:10.1038/nature14408
Published online 03 June 2015
Nature | News & Views:
Cell biology: Nuclear dilemma resolved
Nature (2015) doi:10.1038/nature14527
Published online 03 June 2015
Norske forskarar har avdekka mysterium bak celledeling